Fig. 4

Longitudinal imaging of the cerebral vasculature during the subacute and chronic phase after ischemic stroke. A Maximum intensity projections of two-photon imaged z-stacks of FITC70 signal in M2CA field of view 48 h, 72 h and 7 d post M2CAO, respectively. Black arrow indicates direction of blood flow; yellow arrows indicate vessels with blocked (48 h) and re-established blood flow at transition to chronic phase (72 h/7 d); yellow two-headed arrows indicate putative residual clots; scale bars 100 µm. B, C Maximum intensity projection of M2CA field of view in Claudin5-GFP reporter mice depicting endothelial cells (green) and TRITC70 signal (red) before and after M2CAO; (b', c') depict individual z-planes. White arrows indicate direction of blood flow; arrowheads indicate loop formed in bifurcation (b') and angiogenic sprouting (c'); two-headed arrows indicate intussusceptive pillars; scale bars (B, C, c’) 50 µm; scale bars (b’) 25 µm