Fig. 3
From: Choroid plexus immune cell response in murine hydrocephalus induced by intraventricular hemorrhage
Intraventricular hemorrhage increases the number of ChP macrophages (total and epiplexus), T lymphocytes and neutrophils in CX3CR-1GFPmice. (A) Immunofluorescence staining of ChP Iba-1 in naïve and IVH group at 24 h. Scale bar = 50 μm. (B) Quantification of the number of GFP(+)/Iba-1(+) cells as a percentage of the total GFP(+) cells. Values are expressed as mean ± SD, n = 6 in naive and n = 7 in IVH groups. #p < 0.01 by student t-test. (C) The proportion of epiplexus macrophages to the total number GFP(+) cells was calculated. Values are mean ± SD, n = 6 in naive and n = 7 in IVH groups. #p < 0.01 by student t-test. (D) The percentage of stromal macrophages relative to the total number of GFP(+) cells was calculated. Values are mean ± SD, n = 6 in naive and n = 7 in IVH groups. No significant difference between groups by student t-test. (E) Immunofluorescence staining of ChP CD4 in naïve and IVH groups at 24 h. The first columns scale bar = 50 μm and fourth column scale bar = 20 μm. (F) Calculation of the number of T lymphocytes relative to the number of total ChP cells. Values are medians (25th percentile, 75th percentile), n = 6 in naive and n = 7 in IVH groups. #p < 0.01 by Mann-Whitney U test. (G) Immunofluorescence staining of ChP MPO in naïve and IVH groups at 24 h. The first columns scale bar = 50 μm and fourth column scale bar = 20 μm. (H) Estimation of the number of neutrophils relative to the number of total ChP cells cells. Values are median (25th percentile, 75th percentile), n = 6 in naive and n = 7 in IVH groups. #p < 0.01 by Mann-Whitney U test