Fig. 4
From: Choroid plexus immune cell response in murine hydrocephalus induced by intraventricular hemorrhage
Intraventricular injections of iron and Prx-2 increase ventricular volume, the number and soma size of GFP(+) cells in CX3CR-1GFPmice. (A) Representative T2-weighted MRI scans 24 h after intraventricular injection of saline, iron and Prx-2 into CX3CR-1GFP mice. Note the dilated ventricles in the iron-injected and Prx-2-injected mice. (B) Quantification of ventricular volume. Values are means ± SD, n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups, #p < 0.01 vs. saline group by one-way ANOVA with Tukey’s multiple comparisons test. (C) Fluorescence microscopy of GFP(+) cells and DAPI staining in the ChP of mice injected with saline, iron or Prx-2 at 24 h. Scale bar = 50 μm. (D) The number of GFP(+) cells was calculated as a percentage of the total number of ChP cells (DAPI). Values are means ± SD, n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups. *p < 0.05 for iron vs. saline group and #p < 0.01 for Prx-2 vs. saline group by one-way ANOVA with Tukey’s multiple comparisons test. (E) Fluorescence microscopy of GFP(+) cells and DAPI staining in the ChP of saline-, iron- and Prx-2-injected mice at 24 h. The first columns scale bar = 20 μm and fourth column scale bar = 5 μm. (F) Soma sizes for ChP GFP(+) cells were calculated. Values are means ± SD, n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups. #p < 0.01 vs. saline group by one-way ANOVA with Tukey’s multiple comparisons test