Fig. 5
From: Choroid plexus immune cell response in murine hydrocephalus induced by intraventricular hemorrhage
Intraventricular iron or Prx-2 injection increases the number of ChP macrophages (total and epiplexus), T lymphocytes and neutrophils in CX3CR-1GFPmice. (A) Immunofluorescence staining for ChP Iba-1 in saline-injected, iron-injected and Prx-2-injected mice at 24 h. Scale bar = 50 μm. (B) The number of GFP(+)/Iba-1(+) cells was calculated as a percentage of the total GFP(+) cells. Values are median (25th percentile, 75th percentile), n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups, #p < 0.01 for iron vs. saline group and *p < 0.05 for Prx2 vs. saline group by nonparametric Kruskal-Wallis test with Dunn’s multiple comparisons test. (C) The percentage of epiplexus macrophages was calculated relative to the total number of GFP(+) cells. Values are median (25th percentile, 75th percentile), n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups, *p < 0.05 for iron vs. saline group and #p < 0.01 for Prx2 vs. saline group by nonparametric Kruskal-Wallis test with Dunn’s multiple comparisons test. (D) The percentage of stromal macrophages was calculated relative to the total number of GFP(+) cells. Values are mean ± SD, n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups. No significant differences between groups (one-way ANOVA with Tukey’s multiple comparisons test). (E) Immunofluorescence staining of ChP CD4 in mice injected with saline, iron or Prx-2 at 24 h. Scale bar = 50 μm. (F) The number of T lymphocytes was calculated relative to the number of total ChP cells. Values are means ± SD, n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups. #p < 0.01 vs. saline group by one-way ANOVA with Tukey’s multiple comparisons test. (G) Immunofluorescence staining of ChP MPO in saline-, iron- and Prx-2-injected mice at 24 h. Scale bar = 50 μm. (H) The number of neutrophils was calculated relative to the number of GFP(+) cells. Values are median (25th percentile, 75th percentile), n = 6 in saline, n = 7 in iron and n = 7 in Prx-2 groups. *p < 0.05 for iron vs. saline group and #p < 0.01 for Prx2 vs. saline group by nonparametric Kruskal-Wallis test with Dunn’s multiple comparisons test