Fig. 6
From: Choroid plexus immune cell response in murine hydrocephalus induced by intraventricular hemorrhage
Clodronate treatment decreases ventricular volume, the number of GFP(+) cells and the soma size of GFP(+) cells in CX3CR-1GFPmice. (A) Examples of T2-weighted MRI scans obtained 24 h after intraventricular injection of iron + clodronate liposomes (iron + clo) or iron + control liposomes (iron + con). Note the attenuated ventriculomegaly in the iron + clo group. (B) Quantification of ventricular volume. Values are mean ± SD, n = 6 in iron + con and iron + clo groups, #p < 0.01 by student t-test. (C) Fluorescence images of GFP(+) cells in the ChP of saline + con-injected and iron + clo-injected mice at 24 h. Scale bar = 50 μm. (D) The number of GFP(+) cells was calculated as a percentage of the total number of ChP cells (DAPI). Values are mean ± SD, n = 6, #p < 0.01 by student t-test. (E) Fluorescence imaging of GFP(+) cells within the ChP of mice injected with iron + con and iron + clo at the 24-hour time point. The first columns scale bar = 20 μm and fourth column scale bar = 5 μm. (F) Soma sizes of ChP GFP(+) cells were quantified. Values are mean ± SD, n = 6, #p < 0.01 by student t-test