Fig. 7
From: Choroid plexus immune cell response in murine hydrocephalus induced by intraventricular hemorrhage
Clodronate treatment reduces ChP macrophages (total, epiplexus and stromal), but not the number of T lymphocytes and neutrophils in CX3CR-1GFPmice. (A) Immunofluorescence staining of ChP Iba-1 in iron + control liposomes (con) and iron + clodronate liposomes (clo) groups at 24 h. Scale bar = 50 μm. (B) Calculation of the number of GFP(+)/Iba-1(+) cells (macrophages) as a percentage of total GFP(+) cells. Values are mean ± SD, n = 6, #p < 0.01 by student t-test. The percentage of epiplexus (C) and stromal (D) macrophages was calculated relative to the total number of GFP(+) cells. Values are mean ± SD, n = 6, *p < 0.05 and # p < 0.01 by student t-test. (E) Immunofluorescence staining of ChP CD4 in iron + con and iron + clo group at 24 h. The first columns scale bar = 50 μm and fourth column scale bar = 20 μm. (F) The percentage of T lymphocytes was calculated relative to the total number of ChP cells. Values are mean ± SD, n = 6, no significant difference by student t-test. (G) Immunofluorescence staining of ChP MPO in iron + con and iron + clo group at 24 h. The first columns scale bar = 50 μm and fourth column scale bar = 20 μm. (H) The percentage of neutrophils was calculated relative to the total number of GFP(+) cells. Values are mean ± SD, n = 6, no significant difference by student t-test